(a) Principle of the reactions underlying DDRT-PCR analysis. (N) Any of the four nucleotides G, A, T, or C. V can be any nucleotide besides T. (B) Any nucleotide. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to. Establishment of infection and disease implies modifications in the genetic programmes of the cell systems that are involved and the differential expression of.

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We would like to thank King Khalid University, Faculty of Science, Biology Department for supporting this work with all the required chemicals and equipments. Journal List 3 Biotech v. The obtained sequences were compared with the sequences deposited in GenBank data base http: Eicosane and ethyl acetate extract of Spirulena platensis consisted of heptadecane and tetradecane showed antimicrobial activities against some Gram positive and Gram negative bacteria and Candida albicans Ozdemir et al.

Articles from 3 Biotech are provided here courtesy of Springer. A typical GC—MS chromatogram of the ethanol extracts inhibition zone slices.

The extraction of the active metabolites produced by the endophytic isolate SA was done as follows: In the latter case, please turn on Javascript support in your web browser and reload this page. These results confirm the common concept regarding the ability of microorganism to develop new strategies to resist more and more antibiotics, which invite the researchers to find new and effective antimicrobial agents.

Support Center Support Center. The sterilized cubic stem parts were then divided into two pieces using a sterilized razor blade. The number and extent of the genes affected is not totally known to others.

Differential display – Wikipedia

The protein is well known as antimicrobial agent and was reported to be produced by plants, animals and insects. To investigate the upregulated or downregulated genes during the antagonistic process, both of the pathogenic and the antagonistic bacteria were grown either separately on two different nutrient agar plates or close to each other on the same plate.

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Please review our privacy policy. How does Europe PMC derive its citations network? It also tries to understand the mechanism of action using both of chromatographic and molecular levels. After completion, a fraction of the PCR mixture was examined using 1. This gene was up-regulated, excised from the gel and submitted for gene sequencing Fig. The production of antibiotics and hydrolytic enzymes is a feature of many endophytic bacilli, including Bacillus cereus Pleban et al.

Unveiling of up-regulated or down-regulated genes Any external effect on the organism can affect its genetic behavior in a way of over or down expression of the genes according to the kind drrt the stimulant.

In vitro antifungal activities of inhibitors of phospholipases from the fungal pathogen Cryptococcus neoformans. Cdrt of differential display RT-PCR revealed that the isolate was able to up-regulate a gene with serine protease like protein. A review on production of serine alkaline protease by Bacillus spp.

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Antibacterial activity of volatile component and various extracts of Spirulina platensis. On the other hand, only chloramphenicol recorded 2. Serine protease is also known to be produced by bacteria for purposes oth er than bacterial-bacterial antagonistic effect, which has been confirmed by this study. The antagonistic factor was isolated from the bacterial culture medium and purified by thin layer chromatography technique, then analyzed by GC-MS analysis. Differential display using Ea1 and Ea2 primers combined with OP1 reverse transcription primer.

Verma and Verma reported the isolation and purification of antimicrobial peptide from celomic fluid of Indian earthworm Pheretima posthumous.

The black arrow showed the up-regulated gene of B. However, only one of the sequenced genes was matched with genes deposited in GenBank. Wipat A, Harwood CR. Serine protease is an enzyme that produced by many organisms including bacteria.

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Differential display

Application of differential display RT-PCR revealed that the isolate was able to up-regulate a gene with serine protease like protein. The Fdrt subtilis genome sequence: The obtained sequence revealed that the concerned gene is serine protease-like gene. Detected compounds and its derivatives showed various biological activities, for example, Ganendren et al.

Molecular detection of the antagonistic factor using differential display RT-PCR technique To investigate the upregulated or downregulated genes during the antagonistic process, both of the pathogenic and the antagonistic bacteria were grown either separately on two drrt nutrient agar plates or close to each other on the same plate. Or filter your current search. In vitro antifungal activity of Argemone ochroleuca Sweet latex against some pathogenic fungi.

Open in a separate window. Earthworm- a potential source for stable and potent antimicrobial compounds- isolation and purification study. Helal4, 5 and Sulaiman A.

In-vivo expression technologies and differential display RT-PCR are providing new approaches pc further examine a microbe’s response to experimental conditions which more closely resemble natural microbial associations and habitats. Antagonistic bioactivity of an endophytic bacterium isolated from Epimedium brevicornu Maxim.

Components in the ethanol extract of inhibition zone slices GC—MS chromatogram analysis of the ethanol extract of inhibition zone parts s howed eight peaks that led to the identification of a number of compounds Fig.

Using of ethanol and sodium hypochlorite at certain dilutions is recommended for complete removal of the plant surface bacteria Jan et al. Biocontrol of plant disease: